Major Results

This page contains some, but not all of our major results and highlights of our simulations. This is not complete. More results will be presented at the jamboree.

Simulations: Chemoinduction/Module 1-Output yield of drug of interest

Our simulation results indicate that increasing input amounts of IPTG result in a greater yield of polypeptide drug of interest (See simulation results). We did an experiment to study how different input concentrations of IPTG affect cell growth, and concluded that IPTG has no significant effect on cell growth within the ranges we are using(IPTG growth curves). Therefore, we can conclude that increasing IPTG concentration initially will have a positive contribution to our dosage.

Note 1: Results from these simulations were to be tied up with data from our LacI-RFP testing construct (See our part K200019. Unluckily, this part of the construct was not sent correcty from the registry, so testing again was not possible before the wiki-freeze deadline.
Note 2: Parameters used in the system are arbitrary. For a justification see the system stability analysis.

Experimental: Module 2 - Encapsulation growth curves

Dependent on lab results today

Experimental: Autoinduction - Diauxic growth curve and CRP GFP

Here we can pick a diauxic growth curve and compare directly to a simulation

Simulations: Module 1 - Enzyme kinetics and dosage control

Many polypeptides of interest are enzymes. This means that detecting how much drug has been produced requires knowledge about their interaction with their respective substrates in the enzymatic assays. Here we assumed that enzymatic activity follows Michaelis-Menten kinetics [1-3]. This means that we can directly relate enzymatic activity to the required dosage for successful administration of the polypeptide of interest.
Dosage Calculations:
//upload summary and complete pdf file.

Experimental: Module 3 - Thermoinducible promoter

Need Matthieu's latest processed data

Conclusion

Need some inspiration here guys!

References