"
Team:Imperial College London/Wetlab/Results/Thermoinduction
From 2009.igem.org
(Difference between revisions)
(→42 degrees Celsius) |
(→Experiment Rationale) |
||
| Line 1: | Line 1: | ||
{{Imperial/09/TemplateTop}} | {{Imperial/09/TemplateTop}} | ||
| + | <br><br> | ||
=Experiment Rationale= | =Experiment Rationale= | ||
To investigate the behaviour of the lamda-cI thermoinducible construct and show that when temperature is low (at 28 degrees Celsius), there is low fluorescence output. This shows that the genome deletion module is repressed. | To investigate the behaviour of the lamda-cI thermoinducible construct and show that when temperature is low (at 28 degrees Celsius), there is low fluorescence output. This shows that the genome deletion module is repressed. | ||
When temperature is raised to 42 degrees Celsius, fluorescence increases, indicating that the repression is lifted. | When temperature is raised to 42 degrees Celsius, fluorescence increases, indicating that the repression is lifted. | ||
| - | We are looking at both absorbance and fluorescence data. | + | We are looking at both absorbance and fluorescence data. |
=Absorbance data= | =Absorbance data= | ||
Revision as of 22:44, 20 October 2009
Contents |
Experiment Rationale
To investigate the behaviour of the lamda-cI thermoinducible construct and show that when temperature is low (at 28 degrees Celsius), there is low fluorescence output. This shows that the genome deletion module is repressed. When temperature is raised to 42 degrees Celsius, fluorescence increases, indicating that the repression is lifted. We are looking at both absorbance and fluorescence data.
Absorbance data
Understanding of the fluorescence data requires normalization with cell growth data, in the form of optical density (absorbance).
28 degrees Celsius
Figure 1: Absorbance at 28 degrees Celsius
42 degrees Celsius
Figure 2: Absorbance at 42 degrees Celsius
