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Team:Kyoto/CiC/Goals
From 2009.igem.org
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===Subgoal C --- In Vivo Protein Import into Prepared Liposomes === | ===Subgoal C --- In Vivo Protein Import into Prepared Liposomes === | ||
| - | Our third subgoal is introducing prepared liposomes into host cells and confirming whether they take in mitochondrial proteins form cytoplasm in vivo.This subgoal requires both of subgoals above. | + | Our third subgoal is introducing prepared liposomes into host cells and confirming whether they take in mitochondrial proteins form cytoplasm in vivo.This subgoal requires both of subgoals above.Nobody knows what happens when this sub goal is achieved. |
==Future Goals== | ==Future Goals== | ||
Revision as of 01:34, 21 October 2009
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Goals
In this "cells in cells" project, we set three subgoals.
Subgoal A --- Liposomes that can penetrate through cell membrane
Our first subgoal is construction of liposomes that can penetrate through cell membrane and confirming whether they get into cells.
Subgoal B --- In Vitro Protein Import into Prepared Liposomes
Our second subgoal is construction of prepared liposomes which can take in mitochondrial proteins from cytoplasm and confirming whether they import proteins in vitro.
Subgoal C --- In Vivo Protein Import into Prepared Liposomes
Our third subgoal is introducing prepared liposomes into host cells and confirming whether they take in mitochondrial proteins form cytoplasm in vivo.This subgoal requires both of subgoals above.Nobody knows what happens when this sub goal is achieved.
Future Goals
Well-prepared Liposomes
Construction of double membraned liposomes with all important mitochondrial translocases.
Artificial Mitochondrial Genome
Construction of prepared liposome (or well-prepared liposomes) which have DNA in mitochondrial format.
Chloroplasts
Reconstruction of chloroplasts in similar methods.
