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Team:UNIPV-Pavia/Methods Materials/Glycerol stock
From 2009.igem.org
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== '''Glycerol stocks''' == | == '''Glycerol stocks''' == | ||
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''(estimated time: 15 min + freezing time)'' | ''(estimated time: 15 min + freezing time)'' | ||
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*Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol | *Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol | ||
*Leave at -20°C for one day | *Leave at -20°C for one day | ||
*Move to -80°C the day after | *Move to -80°C the day after | ||
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| + | <br></td><td align='center' valign='bottom' width='300px'> | ||
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| + | *'''Sample of overnight incubated bacteria''' | ||
| + | *'''80% glycerol''' | ||
| + | </td></tr></table></div><br><br><br><br> | ||
| + | </td></tr></table> | ||
Revision as of 11:44, 30 June 2009
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Glycerol stocks
(estimated time: 15 min + freezing time)
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