Team:UNIPV-Pavia/Methods Materials/Glycerol stock

From 2009.igem.org

(Difference between revisions)
(→Glycerol stocks)
(→Glycerol stocks)
Line 46: Line 46:
<table border='1'>
<table border='1'>
<tr><td>
<tr><td>
-
*'''Sample of overnight incubated bacteria'''
+
*'''o/n incubated bacteria'''
*'''80% glycerol'''
*'''80% glycerol'''
</td></tr></table></div><br><br><br><br>
</td></tr></table></div><br><br><br><br>
</td></tr></table>
</td></tr></table>

Revision as of 11:44, 30 June 2009

EthanolPVanimation.gif



Protocols


Glycerol stocks

(estimated time: 15 min + freezing time)

  • Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
  • Leave at -20°C for one day
  • Move to -80°C the day after










  • o/n incubated bacteria
  • 80% glycerol




Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Glycerol_stock"