Team:UNIPV-Pavia/Methods Materials/Digestion
From 2009.igem.org
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*To open vectors: | *To open vectors: | ||
**a volume containing 1 µg of purified plasmid | **a volume containing 1 µg of purified plasmid | ||
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**25 µl final volume | **25 µl final volume | ||
**incubate at 37°C for 2 hours and 30 minutes | **incubate at 37°C for 2 hours and 30 minutes | ||
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+ | <br></td><td align='center' valign='bottom' width='300px'> | ||
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+ | *'''Roche restriction enzymes''' | ||
+ | *'''Roche buffer H''' | ||
+ | *'''Pre-warmed at 37°C bath''' | ||
+ | *'''Cut and gel-extracted vector''' | ||
+ | *'''ddH2O''' | ||
+ | </td></tr></table></div><br><br><br><br> | ||
+ | </td></tr></table> |
Revision as of 11:47, 30 June 2009
BioBrick digestion with restriction enzymes
(estimated time: 3 hours)
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