Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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multipromotor | multipromotor | ||
+ | Three overnights for multipromoter testing were prepared one would be induced by IPTG, other won't be | ||
+ | induced and the latter is a BL21 strain that doesn't have the construction. | ||
+ | |||
+ | For the induction of protein production again we start our culture at .1 abs 620nm and wait until it reaches | ||
+ | .35 abs 620 nm then we add IPTG to a final concentration of .1mM and then | ||
+ | |||
+ | == | ||
Revision as of 05:14, 21 October 2009