Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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I prepared cultures to purify plasmid that contains the parts that we are planning to send to the registry. | I prepared cultures to purify plasmid that contains the parts that we are planning to send to the registry. | ||
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induced and the latter is a BL21 strain that doesn't have the construction. | induced and the latter is a BL21 strain that doesn't have the construction. | ||
- | For the induction of protein production again we start our culture at .1 abs 620nm and wait until it | + | == October 21, 2009 == |
- | .35 abs 620 nm then we | + | |
+ | For the induction of protein production again we start our culture at .1 abs 620nm and wait until it reached | ||
+ | .35 abs 620 nm then we added IPTG to a final concentration of .1mM and then we leave the culture media for four | ||
+ | hours to get some expression from the plasmid that have our contruction. | ||
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== | == |
Revision as of 05:19, 21 October 2009