Team:IIT Bombay India/Notebook

From 2009.igem.org

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[[Week10]] Transformation of 4 strains (Jul, 13-18)
[[Week10]] Transformation of 4 strains (Jul, 13-18)
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[[Week11]] FACS :Constructs (Jul, 20-24)
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[[Week11]] FACS:Constructs (Jul, 20-24)
[[Week12]] FACS (Jul, 27-31)
[[Week12]] FACS (Jul, 27-31)
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[[Week13]] Transformations in ''E.coli'' MG1655 (lac I deleted) (Aug, 3-8)
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[[Week14]] Preparation for time course expression analysis (Aug, 10-15)
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[[Week15]] FACS time course (Aug, 17-22)
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[[Week16]] FACS: different IPTG conc. (Aug, 24-29)
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[[Week17]] Growth curves for all the four strains (Aug-Sep, 31-5)
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[[Week18]] Plasmid extraction and Gel electrophoresis (Sep, 7-13)
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[[Week19]] Growth curve on Lactose (ptet A and plac K) (Sep, 14-20)
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[[Week20]] Growth curve on Lactose (plac A and ptet K) (Sep, 21-27)
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[[Week21]] Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)(Sep-Oct, 28-3)
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[[Week22]] Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)(Oct, 5-10)
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[[Week23]] Growth curve on Lactose (ptet A and plac K) (Oct, 12-17)
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[[Week24]] Data compilation and analysis, wiki uploads(Oct, 18-21)

Revision as of 05:33, 21 October 2009

Week1 FACS Trial

Week2 Preparation (May, 18-23)

Week3 FACS (May, 25-30)

Week4 Growth Curve (Jun, 1-6)

Week5 FACS:YFP signal (Jun, 8-13)

Week6 FACS:CFP signal (Jun, 15-20)

Week7 FACS repeat (Jun, 22-27)

Week8 Req.:Transformation (Jun 29- Jul 4)

Week9 Cultured Host (Jul, 6-11)

Week10 Transformation of 4 strains (Jul, 13-18)

Week11 FACS:Constructs (Jul, 20-24)

Week12 FACS (Jul, 27-31)

Week13 Transformations in E.coli MG1655 (lac I deleted) (Aug, 3-8)

Week14 Preparation for time course expression analysis (Aug, 10-15)

Week15 FACS time course (Aug, 17-22)

Week16 FACS: different IPTG conc. (Aug, 24-29)

Week17 Growth curves for all the four strains (Aug-Sep, 31-5)

Week18 Plasmid extraction and Gel electrophoresis (Sep, 7-13)

Week19 Growth curve on Lactose (ptet A and plac K) (Sep, 14-20)

Week20 Growth curve on Lactose (plac A and ptet K) (Sep, 21-27)

Week21 Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)(Sep-Oct, 28-3)

Week22 Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)(Oct, 5-10)

Week23 Growth curve on Lactose (ptet A and plac K) (Oct, 12-17)

Week24 Data compilation and analysis, wiki uploads(Oct, 18-21)