"
Team:USTC/Modeling/Model-2
From 2009.igem.org
| Line 25: | Line 25: | ||
| - | For a particular concentration of AHL<sub>out</sub>, the value of parameter d could be estimated based on the measurement of the OD. For instance, when the concentration of AHL<sub>out</sub> was zero, the value of d was 0.00368. The values of 4.8E-3 for rmG, 4.8E-3 for a and 0.4 for pG were got from the | + | For a particular concentration of AHL<sub>out</sub>, the value of parameter d could be estimated based on the measurement of the OD. For instance, when the concentration of AHL<sub>out</sub> was zero, the value of d was 0.00368 per minute. The values of 4.8E-3 per sec for rmG, 4.8E-3 per sec for a and 0.4 per sec for pG were got from the experiment conducted by Barry Canton and Anna Labno in 2004, click [http://partsregistry.org/Part:BBa_F2620:Experience/Endy/Data_analysis here] to the detail. |
| - | The values of 4.8E-3 for rmG, 4.8E-3 for a and 0.4 for pG were got from the F2620. | + | The values of 4.8E-3 per sec for rmG, 4.8E-3 per sec for a and 0.4 per sec for pG were got from the F2620. |
[[Image:s-m-2-2.jpg|900px]] | [[Image:s-m-2-2.jpg|900px]] | ||
Revision as of 10:20, 21 October 2009
| Home | Team | Project | Modeling | Parts | Standard & Protocol | Software Tool | Human Practice | Notebook |
|---|
Team:USTC/Modeling/Model-2
Contents |
pConX2+LuxR+pLux/Tet+GFP
Parts: K176026 K176126 K176128 K176130
Simplified Model-2
We employed an ODE model. We defined the input to be AHLout the and the output to be the synthesis rate of mature GFP. In this system,we assumed the concentration of the LuxR was constant.
To simplify the model, the (Recation S2-4), (Recation S2-5) and (Recation S2-4) were considered to be able to get balance in a very short time.
From above equations, we can the change of PoPS respond to the AHLin.
For a particular concentration of AHLout, the value of parameter d could be estimated based on the measurement of the OD. For instance, when the concentration of AHLout was zero, the value of d was 0.00368 per minute. The values of 4.8E-3 per sec for rmG, 4.8E-3 per sec for a and 0.4 per sec for pG were got from the experiment conducted by Barry Canton and Anna Labno in 2004, click [http://partsregistry.org/Part:BBa_F2620:Experience/Endy/Data_analysis here] to the detail.
The values of 4.8E-3 per sec for rmG, 4.8E-3 per sec for a and 0.4 per sec for pG were got from the F2620.
