Team:UNIPV-Pavia/Protocols/Precipitation

From 2009.igem.org

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*Elute with 5-10 µl of ddH2O
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Latest revision as of 10:32, 21 October 2009

EthanolPVanimation.gif



Protocols

DNA precipitation with sodium acetate

(estimated time: 1 hour)

  • Add 1/10 DNA solution volume of sodium acetate 3 M
  • Add 2.5 DNA solution volume of absolute ethanol
  • Freeze at -80°C for 30 min
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Decant supernatant
  • Add 50 µl of 70% ethanol
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Remove all supernatant with a pipette
  • Air dry pellet until ethanol is totally removed
  • Elute with 5-10 µl of ddH2O




INGREDIENTS:

Sodium acetate
Absolute ethanol
Ethanol 70%
ddH2O

DNA sample





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