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Team:Kyoto/CiC/Results
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===Result of Subgoal C=== | ===Result of Subgoal C=== | ||
====sig-GFP expression in HeLa==== | ====sig-GFP expression in HeLa==== | ||
| - | We confirmed the function of the mitochondria | + | We confirmed the function of the signal sequence for importing protein into mitochondria, |
| + | compared the expressioin pattern of sig-GFP or GFP with mitotracker signal. | ||
| + | The GFP signal was detected throughout the cell except for the black granules in the cytoplasm or the nuclear, | ||
| + | while sig-GFP signal showed the string-like pattern in the cytoplasm. The black granules in the cytoplasm observed | ||
| + | in the GFP expressed cell were stained by mitotracker, and the result indicated that the GFP is absent from mito | ||
| + | |||
| + | |||
[[Image:Kyoto_GEDD_1.png|200px|thumb|Fig.1]] | [[Image:Kyoto_GEDD_1.png|200px|thumb|Fig.1]] | ||
In biotechnology, it is a big challenge to control the | In biotechnology, it is a big challenge to control the | ||
</div><!-- /#rightcontents --> | </div><!-- /#rightcontents --> | ||
Revision as of 14:18, 21 October 2009
- Home
- Cells in Cells
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Results & Discussion
Result of Subgoal A
Result of Subgoal B
Result of Subgoal C
sig-GFP expression in HeLa
We confirmed the function of the signal sequence for importing protein into mitochondria, compared the expressioin pattern of sig-GFP or GFP with mitotracker signal. The GFP signal was detected throughout the cell except for the black granules in the cytoplasm or the nuclear, while sig-GFP signal showed the string-like pattern in the cytoplasm. The black granules in the cytoplasm observed in the GFP expressed cell were stained by mitotracker, and the result indicated that the GFP is absent from mito
In biotechnology, it is a big challenge to control the
