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sig-GFP expression in HeLa

We confirmed the function of the signal sequence for importing protein into mitochondria, compared the expressioin pattern of sig-GFP or GFP with mitotracker signal. The GFP signal was detected throughout the cell except for the black granules in the cytoplasm or the nuclear, while sig-GFP signal showed the string-like pattern in the cytoplasm. The black granules in the cytoplasm observed in the GFP-expressing cell were stained by mitotracker, and the result indicated that the GFP was normally eliminated from mitochondria (Fig. NUMBER, GFP and mitotracker merged). In case of sig-GFP, mitochondria stained by a mitotracker and the GFP signal showed almost the same pattern and the yellow color in the merge images (Fig. Number, sig-GFP and mitotracker merged) suggested that the sig-GFP and mitochondria were co-localize in the HeLa cell. We, consequently, our constructed signal sequence has the function of importing protein into mitochondria as expected.