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Team:Valencia/Parts
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We have contributed to the Registry with [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Valencia&Done=1 a set of parts], but one of them outstands all the others: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K222000 aequorin]. This reporter protein needs a chemical compound, coelenterazine, and citoplasmic Ca<sub>2+</sub> to work. This means that light is only emitted whenever the researcher uses coelenterazine and whenever there is a media full of Ca<sub>2+</sub>. There are some works that use aequorin to sense Ca<sub>2+</sub>, but we have tamed the protein to emit light whenever we want with the activation through electric current. | We have contributed to the Registry with [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Valencia&Done=1 a set of parts], but one of them outstands all the others: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K222000 aequorin]. This reporter protein needs a chemical compound, coelenterazine, and citoplasmic Ca<sub>2+</sub> to work. This means that light is only emitted whenever the researcher uses coelenterazine and whenever there is a media full of Ca<sub>2+</sub>. There are some works that use aequorin to sense Ca<sub>2+</sub>, but we have tamed the protein to emit light whenever we want with the activation through electric current. | ||
Revision as of 16:22, 21 October 2009
Our BioBricks
We have contributed to the Registry with [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Valencia&Done=1 a set of parts], but one of them outstands all the others: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K222000 aequorin]. This reporter protein needs a chemical compound, coelenterazine, and citoplasmic Ca2+ to work. This means that light is only emitted whenever the researcher uses coelenterazine and whenever there is a media full of Ca2+. There are some works that use aequorin to sense Ca2+, but we have tamed the protein to emit light whenever we want with the activation through electric current.
A thorough characterization has been made and can be found either at the corresponding page in this wiki or at the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K222000 Registry].
Additionally, we have used a collection of knock outs in order to have genetic negative control that help us confirm that the light effect is not an artifact.
