Team:UNIPV-Pavia/Methods Materials/Glycerol stock

From 2009.igem.org

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*'''o/n or in exp phase bacterial culture'''
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'''o/n or in exp phase bacterial culture'''<hr color='white' width='70%'>
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*'''80% glycerol'''
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'''80% glycerol'''</font>
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Revision as of 08:52, 3 July 2009

EthanolPVanimation.gif



Protocols


Glycerol stocks

(estimated time: 15 min + freezing time)

  • Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol
  • Leave at -20°C for one day
  • Move to -80°C the day after










o/n or in exp phase bacterial culture

80% glycerol





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