Team:TUDelft/Project
From 2009.igem.org
(Difference between revisions)
(→Project Overview) |
|||
Line 8: | Line 8: | ||
===Self-destructive plasmid=== | ===Self-destructive plasmid=== | ||
[[Image:TUDelft_logo.png|50px|right|frame]] | [[Image:TUDelft_logo.png|50px|right|frame]] | ||
+ | Research question: | ||
+ | Can we achieve cell-to-cell communication: | ||
+ | #Using delay device | ||
+ | #Using self-destructive plasmid | ||
+ | #Between two F+ cells | ||
Goals: | Goals: | ||
<br> | <br> |
Revision as of 19:59, 3 July 2009
Page is under construction
Project Overview
Our abstract
Finally TUDelft team has a project!!!
Self-destructive plasmid
Research question: Can we achieve cell-to-cell communication:
- Using delay device
- Using self-destructive plasmid
- Between two F+ cells
Goals:
- Construction and characterization of an inducible or self regulated auto-destructive plasmid
- Tune the differential expression of (at least) two genes within the same plasmid
- Construction of a plasmid which contains the gene of a specific restriction enzyme that, after expression, will destroy the plasmid
- Construction of a plasmid with enough and specific restriction sites which leads to its destruction under restriction enzyme treatment
- Clone and incorporation of the plasmid
Why?
- To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
- To generate a pulse in the expression of a certain gene
- To control the amplitude of the pulse created by the plasmid
- Because it is cool!!!
How?
Not known yet :D
Applications:
- Cell synchronization
- Selectable marker recycling
- Metabolic control analysis
- Recombination
- …
Project Details
The Experiments
Results