"
Team:ArtScienceBangalore/Notebook/Week Four
From 2009.igem.org
(New page: ===June 8th- 17th=== '''What are we trying to do?''' We want the bacteria to lyse, to kill itself from the inside. Well then, to do that we had to get a lysis gene in it. The thing is, ...) |
|||
| Line 1: | Line 1: | ||
| + | <html> | ||
| + | <link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /> | ||
| + | <div id="asMain"> | ||
| + | <div id="asTitle"> | ||
| + | <big><h1>Week Four</h1></big> | ||
| + | </div> | ||
| + | |||
| + | <div id="asMenu"> | ||
| + | <ul> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Team">The Team</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Aproach">Approach</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project">The Project</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach">Outreach</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Links">Links</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery">Gallery</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Parts">Parts</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Safety">Safety</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | </html> | ||
===June 8th- 17th=== | ===June 8th- 17th=== | ||
| Line 43: | Line 65: | ||
File:Failed Ligation.png| The Failed Ligation Attempt | File:Failed Ligation.png| The Failed Ligation Attempt | ||
</gallery> | </gallery> | ||
| + | |||
| + | <html> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | </html> | ||
Revision as of 17:44, 21 October 2009
Week Four
June 8th- 17th
What are we trying to do?
We want the bacteria to lyse, to kill itself from the inside.
Well then, to do that we had to get a lysis gene in it. The thing is, with the lysis gene needs to be turned on by this specific substance called a promoter
There are two kinds of promoters, ones that make the gene they're attached to , be 'on' all the time. Then, you have ones that turn on when you want them to, in our case, when you add a mystery enzyme.
Our Step-wise Process:
How we tried to do this:
Step 1 - Taking dry DNA from wells
Step 2 - Transforming competent cells
Step 3 - Picking a single colony.
Step 4 - Inoculating broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.
Step 5 - Using the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.
Step 6 - Digesting the DNA
Step 7 - Gel Electrophoresis
Step 8- Ligation
Step 9- Transformation and Inoculation
June 9th
Images of the various results attained:
 Agarose gel electrophoresis image of digested K112808 |
 Failure 1 |
 The pBAD Insert |
 The Failed Ligation Attempt |
