Team:NTU-Singapore/Project/Wetlab

(Difference between revisions)

Revision as of 17:48, 21 October 2009

Welcome to the project page!

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pLaqUe Out! Research Approach Sensing Device « Degradation Device « Imaging Device « Wetlab Highlights [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=NTU-Singapore Parts Submitted] References

Conclusive Results

The following are the constructs that we have shown to be working.

The first construct is a system construct, in that it was intended for our system to feature this as a functional part.

The second construct is a characterization construct to characterize the modular function of the J23119 promoter in the transcription of NorR.

The third construct is also a test construct to only test and characterize the enzymatic activity of CHE.

The fourth construct is a test construct to only test the reporting ability of IFP.

NTU@iGEMcc 2009. Some rights reserved.

@@ Line 32: / Line 32: @@
 '''The following are the constructs that we have shown to be working.'''
-# J23119-RBS-NorR-Term (''system construct'')
+# J23119-B0034-NorR-B0015 (''system construct'')
-# J23119-RBS-GFP-Term (''characterization construct'')
+# J23119-B0034-GFP-B0015 (''characterization construct'')
-# pLacI-RBS-CHE-Term (''test construct'')
+# pLacI-B0034-CHE-B0015 (''test construct'')
-# J23199-RBS-HO1-Term-J23119-RBS-IFP1.4-Term (''test construct'')
+# J23199-B0034-HO1-B0015-J23119-B0034-IFP1.4-B0015 (''test construct'')
+The first construct is a system construct, in that it was intended for our system to feature this as a functional part.
+The second construct is a characterization construct to characterize the modular function of the J23119 promoter in the transcription of NorR.
+The third construct is also a test construct to only test and characterize the enzymatic activity of CHE.
+The fourth construct is a test construct to only test the reporting ability of IFP.
-The first construct