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Team:NTU-Singapore/Notebook
From 2009.igem.org
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| - | <u>''' | + | <u>'''1st July'''</u> |
| - | * | + | * PE for plaqIQ, J23101, J23119, B0034(rbs), B0015(Term-T for short), B0034-GFP-B0015 |
| - | <u>''' | + | <u>'''2nd July'''</u> |
| - | * | + | * PE for pNO, IFP, CHE |
| + | * DD of pNO, J23119, J23101, rbs-GFP-T | ||
| + | * Our first ligation! | ||
| - | |||
| - | |||
| + | <u>'''3rd July'''</u> | ||
| + | * DD of CHE, IFP and B0015, plaqIQ and plux. | ||
| + | * Ligation. | ||
| - | <u>''' | + | |
| - | * | + | <u>'''4th July'''</u> |
| + | * PE: pNO-GFP; J23101-GFP; J23119-GFP; HO1 | ||
| + | * DD: HO1 | ||
<u>''' '''</u> | <u>''' '''</u> | ||
Revision as of 19:10, 21 October 2009
Experiment Timeline
June
18th June
- Preparation of LB medium
- Amplification of parts from registry (pTet, TetR, HO1, Low copy plasmid)
19th June
- Antibiotic stock preparation
- Inoculation
20th June
- Our first Plasmid Extraction (PE)!
22nd June
- First Double Digestion (DD) and Gel Check!
23rd June
- PE & DD of the registry constructs….
24th June
- Our direct synthesized parts finally arrived. We now have CHE (cholesterol esterase), IFP(Infrared protein 1.4) and pNO (NO sensing promoter).
26th June
- PE , DD and Gel check for the direct synthesized gene sequence.
27th June
- Gel Check for CHE, IFP and pNO
July
1st July
- PE for plaqIQ, J23101, J23119, B0034(rbs), B0015(Term-T for short), B0034-GFP-B0015
2nd July
- PE for pNO, IFP, CHE
- DD of pNO, J23119, J23101, rbs-GFP-T
- Our first ligation!
3rd July
- DD of CHE, IFP and B0015, plaqIQ and plux.
- Ligation.
4th July
- PE: pNO-GFP; J23101-GFP; J23119-GFP; HO1
- DD: HO1
