Team:Alberta/Project/Glycerol Stock

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<ul><li> If we make extra we have them if we need to send them out or want to store in several locations.</ul>
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<ul><li> If we make extra, we have them if we need to send them out or want to store in several locations.</ul>

Revision as of 19:25, 21 October 2009

University of Alberta - BioBytes










































































































Glycerol Stock

What you will need:

  • Overnight with bacterial growth
  • Screw cap tubes
  • Glycerol

Procedure:

  • Pipet 150 uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
  • Add 350 uL of overnight culture to each tube.
  • Pipet up and down to gently mix.
  • Flash freeze in liquid N2 or dry ice/methanol bath
  • Place in -80 C freezer when frozen.

Notes

  • If we make extra, we have them if we need to send them out or want to store in several locations.

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