Team:Alberta/Project/Gel Extraction

From 2009.igem.org

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     <li>Place the gel on the transilluminator. Put on face shield and gloves.
     <li>Place the gel on the transilluminator. Put on face shield and gloves.
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     <li>urn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
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     <li>Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
     <li>Pry out the gel ‘cube’ and place it in an eppendorf tube.
     <li>Pry out the gel ‘cube’ and place it in an eppendorf tube.
     <li>Follow instructions in kit manual.  
     <li>Follow instructions in kit manual.  

Latest revision as of 19:44, 21 October 2009

University of Alberta - BioBytes










































































































Gel Extraction

What you will need:

  • Clean scalpel
  • Transilluminator
  • Eppendorf tube
  • Gel extraction kit
  • 55degC water bath
  • Vortex
  • Microfuge

Procedure

  • Place the gel on the transilluminator. Put on face shield and gloves.
  • Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
  • Pry out the gel ‘cube’ and place it in an eppendorf tube.
  • Follow instructions in kit manual.

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