Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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For the induction of protein production again we started our culture at .1 abs 620nm in 100mL LB and waited until it reached 0.35 abs 620 nm then we added IPTG to a final concentration of 0.1mM and then we leave the culture media for four | For the induction of protein production again we started our culture at .1 abs 620nm in 100mL LB and waited until it reached 0.35 abs 620 nm then we added IPTG to a final concentration of 0.1mM and then we leave the culture media for four | ||
- | hours to see if the GFP that is under the control of the multipromotr | + | hours to see if the GFP that is under the control of the multipromotr get expressed. |
- | Using microscope with suitable filters and light to see GFP we got the following pictures form the IPTG induced BL21 strain that contained our construction | + | Using a microscope with suitable filters and light to see GFP we got the following pictures form the IPTG induced BL21 strain that contained our construction and the BL21 with our construction but without IPTG inductor; BL21 that doesn't have |
- | + | our construction didn't fluoresce at all so it was impossible to take a picture with the micro that we were using. | |
+ | This results support the functioning of multipromoter in particular for T7 polymerase | ||
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Revision as of 19:49, 21 October 2009