Team:Imperial College London/Major results

From 2009.igem.org

(Difference between revisions)
(Module 3 - Genome Deletion)
(Module 3 - Genome Deletion)
Line 13: Line 13:
*The wet lab has also investigated the [[Team:Imperial_College_London/Wetlab/Results/RestrictionDigest | effects of restriction enzymes on cell population]]. Positive results were achieved.  
*The wet lab has also investigated the [[Team:Imperial_College_London/Wetlab/Results/RestrictionDigest | effects of restriction enzymes on cell population]]. Positive results were achieved.  
-
[[Image:II09_DpnII_Digest.png |400px |left]][[Image:II09_TaqI_Digest.png | 400px |right]]
+
[[Image:II09_DpnII_Digest.png |400px][[Image:II09_TaqI_Digest.png | 400px]]
 +
The Dam negative strains are fully cleaved at all different concentrations of restriction enzyme. This produces the 'smear' that can be seen, as the DNA fragments are all of varying, but short lengths
From these results, we have shown that restriction enzymes can cleave Dam negative strains, hence killing Dam negative cells. This also proves Dam positive strains are protected again genomic deletion.
From these results, we have shown that restriction enzymes can cleave Dam negative strains, hence killing Dam negative cells. This also proves Dam positive strains are protected again genomic deletion.

Revision as of 21:01, 21 October 2009

Contents

Major Results

This page contains highlights of our major results and simulations. It is by no means complete. However, more results will be presented at the jamboree.

Module 2 - Encapsulation

Wet lab experiments were performed to investigate growth of E. Coli cells under environments of different pH.

Module 3 - Genome Deletion

[[Image:II09_DpnII_Digest.png |400px]II09 TaqI Digest.png

The Dam negative strains are fully cleaved at all different concentrations of restriction enzyme. This produces the 'smear' that can be seen, as the DNA fragments are all of varying, but short lengths

From these results, we have shown that restriction enzymes can cleave Dam negative strains, hence killing Dam negative cells. This also proves Dam positive strains are protected again genomic deletion.



cell death model

Module Integration - Autoinduction

crp promoter

diauxic growth

Module Integration - Thermoinduction

hvd gfp


References

[1]Physica A: Statistical and Theoretical Physics, Vol. 188, No. 1-3. (1 September 1992), pp. 404-425. A rigorous derivation of the chemical master Equation

Mr. Gene   Geneart   Clontech   Giant Microbes