Team:Heidelberg/Project Further

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(→Further Subprojects)
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* We studied, cloned and characterized some [[Team:Heidelberg/Project_Natural_promoters|natural promoters]]
* We studied, cloned and characterized some [[Team:Heidelberg/Project_Natural_promoters|natural promoters]]
* We created a preliminary [[Team:Heidelberg/stables|stable cell line]] containing a FRT site(s).
* We created a preliminary [[Team:Heidelberg/stables|stable cell line]] containing a FRT site(s).
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* We attempted to create a dual assay plasmid for promoter measurement, which should have the advantage of two fluorescent proteins with different promoters. One of them would be constitutive the other one to be tested. This construction would allow a standardized comparison of promoter strength, due to the elimination of different transfection rates.  
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* We attempted to create a dual assay plasmid for promoter measurement, which should have the advantage of two fluorescent proteins with different promoters. One of them would be constitutive the other one to be tested. This construction would allow a standardized comparison of promoter strength, due to the elimination of different transfection efficiencies. Unfortunately the repeated cloning of the construct, which is shown in Fig 1, was not successful.
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Unfortunately the repeated cloning of the construct in Fig 1. was not successful.
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[[Image:Dual_plasmid.png|thumb|left|300px|<div style="text-align:justify;">'''Fig. 1: The planned dual assay plasmid contained a site for promoter exchange, where one can put the promoter, that has to be tested. This promoter is upstream of a GFP gene and regulates its transcription. This gene is followed by a Jet promoter that constitiutively promotes transcription of a mCherry, serving as a reference.</div>]]
|width="250px" style="padding: 0 20px 15px 15px; background-color:#d8d5d0"|
|width="250px" style="padding: 0 20px 15px 15px; background-color:#d8d5d0"|
|}
|}

Revision as of 21:55, 21 October 2009

Further Subprojects

  • We studied, cloned and characterized some natural promoters
  • We created a preliminary stable cell line containing a FRT site(s).
  • We attempted to create a dual assay plasmid for promoter measurement, which should have the advantage of two fluorescent proteins with different promoters. One of them would be constitutive the other one to be tested. This construction would allow a standardized comparison of promoter strength, due to the elimination of different transfection efficiencies. Unfortunately the repeated cloning of the construct, which is shown in Fig 1, was not successful.


Fig. 1: The planned dual assay plasmid contained a site for promoter exchange, where one can put the promoter, that has to be tested. This promoter is upstream of a GFP gene and regulates its transcription. This gene is followed by a Jet promoter that constitiutively promotes transcription of a mCherry, serving as a reference.
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