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Team:UAB-Barcelona/Primersn
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Revision as of 22:00, 21 October 2009
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These were the primers we ordered for the nitrite: • Pnir: EcoR1-Not-Xba— nirk – Spe Reverse: S
GTCAACGT ACTAGT CTGCACGAAAGACCTTTAAATAC Temperature annealing = 62 ºC
Forward: E N X
CAGTAGTA GAATTC GCGGCCGC TCTAGA GTTGCGCGAAATACCGTAAC Temperature annealing= 60 ºC
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