Virginia Commonwealth/4 September 2009
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==Friday 4 September 2009== | ==Friday 4 September 2009== | ||
+ | ==Thursday 10 September 2009== | ||
===Results=== | ===Results=== | ||
- | * 1 | + | * Data for a 40x Concentration of DNA: |
- | + | ||
+ | {| cellpadding="5" border="1" padding="5" | ||
+ | |+ | ||
+ | |- | ||
+ | ! Promoter!! A260 !! Concentration !! Volume | ||
+ | |- | ||
+ | ! J23102 | ||
+ | | 0.068 || 136 || 40 | ||
+ | |- | ||
+ | ! J23101 | ||
+ | | 0.020 || 40 || 45 | ||
+ | |- | ||
+ | ! pSB1C3 | ||
+ | | 0.047 || 94 || 37 | ||
+ | |- | ||
+ | ! J06702 | ||
+ | | 0.035 || 70 || 41 | ||
+ | |} | ||
+ | |||
---- | ---- | ||
===Tasks=== | ===Tasks=== | ||
- | * | + | * Perform digestion, ligation and run a gel of J23101, J23102, J06702 and pSB1C3. |
- | + | ||
---- | ---- | ||
====Wetlab==== | ====Wetlab==== | ||
- | * | + | * Digestion and ligation of: J23101, J23102, J06702 and pSB1C3. |
- | * | + | *Transformed into cells. |
Latest revision as of 22:56, 21 October 2009
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Contents |
Friday 4 September 2009
Thursday 10 September 2009
Results
- Data for a 40x Concentration of DNA:
Promoter | A260 | Concentration | Volume |
---|---|---|---|
J23102 | 0.068 | 136 | 40 |
J23101 | 0.020 | 40 | 45 |
pSB1C3 | 0.047 | 94 | 37 |
J06702 | 0.035 | 70 | 41 |
Tasks
- Perform digestion, ligation and run a gel of J23101, J23102, J06702 and pSB1C3.
Wetlab
- Digestion and ligation of: J23101, J23102, J06702 and pSB1C3.
- Transformed into cells.