"
Team:Calgary/Lab/Chassis Selection
From 2009.igem.org
(Difference between revisions)
Jamie.feng (Talk | contribs) |
Jamie.feng (Talk | contribs) |
||
| Line 71: | Line 71: | ||
<div class="heading">CHASSIS SELECTION</div> | <div class="heading">CHASSIS SELECTION</div> | ||
<div class="desc"> | <div class="desc"> | ||
| - | <img src="http://i1001.photobucket.com/albums/af132/igemcalgary/l.gif" align="left" hspace=10> | + | <img src="http://i1001.photobucket.com/albums/af132/igemcalgary/l.gif" align="left" hspace=10><br> |
| - | + | ||
In order to test and characterize the AI-2 system, AI-2 was isolated from Vibrio harveyi. Vibrio harveyi MM32 (BB120 luxN::Tn5 luxS::Tn5)<sup>1</sup> is an ideal reporter strain as it cannot respond to AHL nor synthesize AI-2, respectively. In addition, since E. coli produce AI-2, we need to set our system up in cells that do not produce AI-2 (i.e. DH5α cells) and thus, we need to verify that these cells do not produce AI-2. | In order to test and characterize the AI-2 system, AI-2 was isolated from Vibrio harveyi. Vibrio harveyi MM32 (BB120 luxN::Tn5 luxS::Tn5)<sup>1</sup> is an ideal reporter strain as it cannot respond to AHL nor synthesize AI-2, respectively. In addition, since E. coli produce AI-2, we need to set our system up in cells that do not produce AI-2 (i.e. DH5α cells) and thus, we need to verify that these cells do not produce AI-2. | ||
| + | |||
| + | </html> | ||
| + | |||
| + | |||
<html> | <html> | ||
Revision as of 01:39, 22 October 2009
UNIVERSITY OF CALGARY
|
LAB INDEX
|
CHASSIS SELECTION
 In order to test and characterize the AI-2 system, AI-2 was isolated from Vibrio harveyi. Vibrio harveyi MM32 (BB120 luxN::Tn5 luxS::Tn5)1 is an ideal reporter strain as it cannot respond to AHL nor synthesize AI-2, respectively. In addition, since E. coli produce AI-2, we need to set our system up in cells that do not produce AI-2 (i.e. DH5α cells) and thus, we need to verify that these cells do not produce AI-2.
|
