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Team:Calgary/Lab/Chassis Selection
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<img src="http://i1001.photobucket.com/albums/af132/igemcalgary/l.gif" align="left" hspace=10><br> | <img src="http://i1001.photobucket.com/albums/af132/igemcalgary/l.gif" align="left" hspace=10><br> | ||
In order to test and characterize the AI-2 system, AI-2 was isolated from Vibrio harveyi. Vibrio harveyi MM32 (BB120 luxN::Tn5 luxS::Tn5)<sup>1</sup> is an ideal reporter strain as it cannot respond to AHL nor synthesize AI-2, respectively. In addition, since E. coli produce AI-2, we need to set our system up in cells that do not produce AI-2 (i.e. DH5α cells) and thus, we need to verify that these cells do not produce AI-2. | In order to test and characterize the AI-2 system, AI-2 was isolated from Vibrio harveyi. Vibrio harveyi MM32 (BB120 luxN::Tn5 luxS::Tn5)<sup>1</sup> is an ideal reporter strain as it cannot respond to AHL nor synthesize AI-2, respectively. In addition, since E. coli produce AI-2, we need to set our system up in cells that do not produce AI-2 (i.e. DH5α cells) and thus, we need to verify that these cells do not produce AI-2. | ||
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[[Image:Calgary_Tour1t.png]][[Image:Calgary_Toure.png]][[Image:Calgary_Touro.png]][[Image:Calgary_Toure.png]][[Image:Calgary_Touro.png]][[Image:Calgary_Toure.png]] | [[Image:Calgary_Tour1t.png]][[Image:Calgary_Toure.png]][[Image:Calgary_Touro.png]][[Image:Calgary_Toure.png]][[Image:Calgary_Touro.png]][[Image:Calgary_Toure.png]] | ||
Revision as of 01:53, 22 October 2009
UNIVERSITY OF CALGARY
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LAB INDEX
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CHASSIS SELECTION
 In order to test and characterize the AI-2 system, AI-2 was isolated from Vibrio harveyi. Vibrio harveyi MM32 (BB120 luxN::Tn5 luxS::Tn5)1 is an ideal reporter strain as it cannot respond to AHL nor synthesize AI-2, respectively. In addition, since E. coli produce AI-2, we need to set our system up in cells that do not produce AI-2 (i.e. DH5α cells) and thus, we need to verify that these cells do not produce AI-2.
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