Team:Harvard/Laser
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- | <b>Problems with cell age</b> In our experiments, we found that whether or not the assay worked is highly dependent on cell age. In the following experiment, we grew cells in liquid medium with PCB in the dark (or DMSO for control), then pipetted an aliquot of cells into each well of a 96 well plate. Each well was exposed to red laser light for the indicated amount of time, and then covered and allowed to incubate for 3 hours. After the incubation period the wells were swabbed with q-tips, which were then dipped in liquid nitrogen to lyse the cells, and then submerged in x-gal solution. Q-tips with cells producing beta gal turned blue. < | + | <b>Problems with cell age</b> In our experiments, we found that whether or not the assay worked is highly dependent on cell age. In the following experiment, we grew cells in liquid medium with PCB in the dark (or DMSO for control), then pipetted an aliquot of cells into each well of a 96 well plate. Each well was exposed to red laser light for the indicated amount of time, and then covered and allowed to incubate for 3 hours. After the incubation period the wells were swabbed with q-tips, which were then dipped in liquid nitrogen to lyse the cells, and then submerged in x-gal solution. Q-tips with cells producing beta gal turned blue. <br> |
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Revision as of 02:50, 22 October 2009
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