Team:USTC/Modeling/Model-11

From 2009.igem.org

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From above equations, we can the change of PoPS respond to the AHL<sub>in</sub>.
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In above equations, all parameters could be obtained from our previous models. For instance, the kkk was estiamted in the [[Team:USTC/Modeling/Model-3|'''Simplified Model-3''']], the kk was calculated in the [[Team:USTC/Modeling/Model-2|'''Simplified Model-2''']], and the k<sub>21</sub>, rAHL<sub>in</sub> were available in [[Team:USTC/Modeling/Model-5|'''Simplified Model-5''']]. The PoPS<sub>cI</sub> cloud be calculated in [[Team:USTC/Modeling/Model-6|'''Simplified Model-6''']], dependenting on the PoPS before it.
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[[Image:sm2-2.jpg|600px]]  
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For this system, the value of parameter d, 0.004576, could be estimated based on the measurement of the OD.
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The values of C<sub>1</sub>, C<sub>2</sub>, C<sub>3</sub>, C<sub>4</sub>, k3, and rAHL were same to be those of model-2, click [[Team:USTC/Modeling/Model-2|'''here''']] to the '''Simplified Model-2'''.
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The values of 4.8E-3 per sec for rmG, 1.8E-3 per sec for a and 0.4 per sec for pG were got from the experiment conducted by Barry Canton and Anna Labno in 2004, click [http://partsregistry.org/Part:BBa_F2620:Experience/Endy/Data_analysis here] to the details.
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[[Image:SM5-3.jpg|450px]]
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Revision as of 03:05, 22 October 2009

USTC
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Team:USTC/Modeling/Model-11

Contents


pConX3+mRFP+cI+pcI+LuxI+tetR+pConX1+LuxI+pConX2+LuxR+pLux/Tet+ccdB

Parts:


Model-11-1.jpg

Model-11-2.jpg

Model-11-3.jpg


Simplified Model-11

To get the datails of the model-processing, click Simplified Model-2

We employed an ODE model. In this system,we assumed the synthesis rate of TetR, LuxR, cI and LuxI were totally decided by the Promoter before them, and the concentration of them were constant.

Sm-11-1.jpg


To simplify the model, the (Recation S11-4), (Recation S11-5), (Recation S11-10), (Recation S11-12), (Recation S11-14), (Recation S11-15), (Recation S11-16), (Recation S11-17) and (Recation S11-18) were considered to be able to get balance in a very short time.


Sm11-2.jpg


In above equations, all parameters could be obtained from our previous models. For instance, the kkk was estiamted in the Simplified Model-3, the kk was calculated in the Simplified Model-2, and the k21, rAHLin were available in Simplified Model-5. The PoPScI cloud be calculated in Simplified Model-6, dependenting on the PoPS before it.

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