LB Broth

From 2009.igem.org

(Difference between revisions)
(LB Growth Media)
(LB Growth Media)
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*5g yeast extract
*5g yeast extract
*10g Bacto-tryptone
*10g Bacto-tryptone
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 +
If you are planning on adding an antibiotic add them after the media has cooled to around 50 degrees or less. Label each container of media. Example: "LB+AMP 100ug/mL 6/10/09".

Revision as of 20:20, 10 July 2009

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LB Growth Media

  • 1L dH20
  • 10g NaCl
  • 5g yeast extract
  • 10g Bacto-tryptone

If you are planning on adding an antibiotic add them after the media has cooled to around 50 degrees or less. Label each container of media. Example: "LB+AMP 100ug/mL 6/10/09".