Team:TUDelft/Literature
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Revision as of 12:08, 12 July 2009
Page is under construction
Project Overview
Contents |
Our abstract
Finally TUDelft team has a project!!!
Self-destructive plasmid
Research question: Can we achieve cell-to-cell communication:
- Using delay device
- Using self-destructive plasmid
- Between two F+ cells
Goals:
- Build a delay device that can change the timing of transcribing the target protein
- Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)
- Construct a modified F plasmid that will allow F+/F+ conjugation
Why?
- To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
- To generate a pulse in the expression of a certain gene
- To control the amplitude of the pulse created by the plasmid
- Because it is cool!!!
How?
Not known yet :D
Applications:
- Cell synchronization
- Selectable marker recycling
- Metabolic control analysis
- Recombination
- …
Project Details
The Experiments
Results