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Virginia Commonwealth/17 July 2009
From 2009.igem.org
(Difference between revisions)
(→Results) |
(→Tasks) |
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| Line 10: | Line 10: | ||
===Tasks=== | ===Tasks=== | ||
''Maria and Afton'' | ''Maria and Afton'' | ||
| - | * Religate promoters that did not grow in the first transformation | + | * Religate promoters that did not grow in the first transformation (backbone pSB4C5) |
| + | ** J23101 w/ E0240 | ||
| + | ** J23105 w/ E0240 | ||
* Run an electrophoresis test on J06702 and pSB4C5 | * Run an electrophoresis test on J06702 and pSB4C5 | ||
| + | [[User:Trentay|Trentay]] 15:53, 17 July 2009 (UTC) | ||
---- | ---- | ||
====Wetlab==== | ====Wetlab==== | ||
''Maria and Afton'' | ''Maria and Afton'' | ||
| - | * Cryogenic stock of the following BioBricks was made | + | * Cryogenic stock of the following BioBricks was made (2 vials each) |
** J23101 w/ J06702 | ** J23101 w/ J06702 | ||
** J23102 w/ E0240 | ** J23102 w/ E0240 | ||
| Line 27: | Line 30: | ||
** J23107 w/ E0240 | ** J23107 w/ E0240 | ||
** J23107 w/ J06702 | ** J23107 w/ J06702 | ||
| + | * Electrophoresis test | ||
| + | [[User:Trentay|Trentay]] 15:53, 17 July 2009 (UTC) | ||
Revision as of 15:53, 17 July 2009
Contents |
Thursday 16 July 2009
Results
- Results of overnight culture was successful
- NEB 10 beta electrocompetency was successful
- NEB on LB had lawn growth
- NEB on LB after being shocked had lawn growth
Trentay 15:50, 17 July 2009 (UTC)
Tasks
Maria and Afton
- Religate promoters that did not grow in the first transformation (backbone pSB4C5)
- J23101 w/ E0240
- J23105 w/ E0240
- Run an electrophoresis test on J06702 and pSB4C5
Trentay 15:53, 17 July 2009 (UTC)
Wetlab
Maria and Afton
- Cryogenic stock of the following BioBricks was made (2 vials each)
- J23101 w/ J06702
- J23102 w/ E0240
- J23102 w/ J06702
- J23103 w/ E0240
- J23103 w/ J06702
- J23104 w/ E0240
- J23104 w/ J06702
- J23105 w/ J06702
- J23107 w/ E0240
- J23107 w/ J06702
- Electrophoresis test
Trentay 15:53, 17 July 2009 (UTC)
