"
Uppsala-Sweden/15 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: '''Determination of linear range OD culture measurement.''' Start culture was diluted 1 2 4 8 16 32 64 128 parts to final volume 200 µl on 96-well plate with BG-11 growth media, triplica...) |
|||
| Line 3: | Line 3: | ||
Start culture was diluted 1 2 4 8 16 32 64 128 parts to final volume 200 µl on 96-well plate with BG-11 growth media, triplicates and blank (BG-11) used. | Start culture was diluted 1 2 4 8 16 32 64 128 parts to final volume 200 µl on 96-well plate with BG-11 growth media, triplicates and blank (BG-11) used. | ||
| - | With lid the corrected (against background) linear range was OD 0,334 - 0,042, with lid OD 0,430 - 0,060. | + | With lid the corrected (against background) linear range was OD 0,334 - 0,042, with out lid OD 0,430 - 0,060. |
Note: the true linear range is larger but the values above are using the existing dilutions as end points. | Note: the true linear range is larger but the values above are using the existing dilutions as end points. | ||
/Anders | /Anders | ||
Latest revision as of 12:03, 19 July 2009
Determination of linear range OD culture measurement.
Start culture was diluted 1 2 4 8 16 32 64 128 parts to final volume 200 µl on 96-well plate with BG-11 growth media, triplicates and blank (BG-11) used.
With lid the corrected (against background) linear range was OD 0,334 - 0,042, with out lid OD 0,430 - 0,060.
Note: the true linear range is larger but the values above are using the existing dilutions as end points.
/Anders
