Uppsala-Sweden/21 July 2009

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Revision as of 11:40, 23 July 2009

Butanol survival experiment

Goal: Measure isobutanol tolerance of Synecocystis. Will be done using triplicates of isobutanol in E-flasks.

Volumes: 15 ml final for E-flask. Concentrations: 8 2, 1, 0,5 , 0,25 , 0 vol% percent isobutanol.

Growth media: BG-11, nitrate supplemented as dictated by standard protocol.

11:00 OD measurement of start culture. OD found to be at 0,273.., within linear range as established 09-07-16 (OD 0,334-0,042) --Anders 11:40, 23 July 2009 (UTC)

excel sheet Media:Butanol survival test conc Plate.xls

--Karl.brune 16:04, 20 July 2009 (UTC)

@@ Line 1: / Line 1: @@
 {{Uppsala-Sweden Template}}
+'''Butanol survival experiment'''
+Goal: Measure isobutanol tolerance of Synecocystis. Will be done using triplicates of isobutanol in E-flasks.
+Volumes: 15 ml final for E-flask. Concentrations: 8 2, 1, 0,5 , 0,25 , 0 vol% percent isobutanol.
+Growth media: BG-11, nitrate supplemented as dictated by standard protocol.
+:00 OD measurement of start culture. OD found to be at 0,273.., within linear range as established 09-07-16 (OD 0,334-0,042)
+--[[User:Anders|Anders]] 11:40, 23 July 2009 (UTC)
 excel sheet [[Media:Butanol survival test conc Plate.xls]]
 --[[User:Karl.brune|Karl.brune]] 16:04, 20 July 2009 (UTC)