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Team:British Columbia/Project
From 2009.igem.org
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== Logic Gates == | == Logic Gates == | ||
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| + | === Miscellaneous Data === | ||
| + | [[Media:Biobricks.zip|Biobricks.zip]] - Fasta file containing every biobrick from [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=List Here] | ||
Revision as of 17:44, 15 May 2009
| You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. |  |
|
Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs) |  |
| Team Example 2 |
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Sponsor Us! | Calendar | Biosensor Sensitivity Notebook | Biosensor Logic Gate Notebook |
|---|
Contents |
Overall project
1. Sensitivity of a biosensor
2. Logic gates
Biosensor
Using a random mutagenesis method directed at the promoter binding sites, we intend to construct a library of varying strength pBAD promoters coupled to a GFP reporter gene. Sequencing of select mutated promoters will result in a collection of pBAD variants that cover a wide range promoter sensitivity.
The Experiments
1. Random mutagenesis of pBAD promoter sequence. 2. Quantification of mutant promoter-driven GFP fluorescence. 3. Sequencing of select mutants. 4. BioBrick design and submission.
Results
Logic Gates
Miscellaneous Data
Biobricks.zip - Fasta file containing every biobrick from [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=List Here]
