Team:British Columbia/Project

From 2009.igem.org

(Difference between revisions)

Revision as of 22:06, 15 May 2009

	Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Sponsor Us!	Biosensor Sensitivity Notebook	Biosensor Logic Gate Notebook

Overall project

1. Sensitivity of a biosensor

2. Logic gates

Biosensor

Using a random mutagenesis method directed at the promoter binding sites, we intend to construct a library of varying strength pBAD promoters coupled to a GFP reporter gene. Sequencing of select mutated promoters will result in a collection of pBAD variants that cover a wide range promoter sensitivity.

The Experiments

1. Random mutagenesis of pBAD promoter sequence. 2. Quantification of mutant promoter-driven GFP fluorescence. 3. Sequencing of select mutants. 4. BioBrick design and submission.

Results

Logic Gates

Miscellaneous Data

Biobricks.zip - Fasta file containing every biobrick from [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=List Here]

Links

http://mobyle.pasteur.fr/cgi-bin/portal.py - a set of web-accessible bioinformatics tools including Mfold, which determines 2D RNA structure and draws it

http://frodo.wi.mit.edu/ - Primer3, a primer design program

@@ Line 1: / Line 1: @@
-{|align="justify"
+{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
-|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+![[Image:IGEMFinalGoldDNA-logo.jpg|100x100px]]
-|[[Image:Example_logo.png|200px|right]]
+!align="center"|[[Team:British_Columbia|Home]]
-|-
+!align="center"|[[Team:British_Columbia/Team|The Team]]
-|
+!align="center"|[[Team:British_Columbia/Project|The Project]]
-''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+!align="center"|[[Team:British_Columbia/Parts|Parts Submitted to the Registry]]
-|[[Image:Team.png|right]]
+!align="center"|[[Team:British_Columbia/Modeling|Modeling]]
-|-
+!align="center"|[[Team:British_Columbia/Sponsor_Us|Sponsor Us!]]
-|
+!align="center"|[[Team:British_Columbia/Biosensor_Sensitivity|Biosensor Sensitivity Notebook]]
-|align="center"|[[Team:British_Columbia | Team Example 2]]
+!align="center"|[[Team:British_Columbia/Biosensor_Logic_Gate|Biosensor Logic Gate Notebook]]
 |}
 <!--- The Mission, Experiments --->
-{{Team:British_Columbia/Templates/Header}}
 == '''Overall project''' ==