Team:Calgary/20 May 2009

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AMPLIFICATION OF ''luxCDABE'' USING Taq POLYMERASE
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WIKI CODING HERE:
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THE PURPOSE OF THIS EXPERIMENT WAS TO AMPLIFY ''LUXCDABE'' WITH TAQ POLYMERASE. GENE SPECIFIC ''LUXCDABE'' FORWARD AND REVERSE PRIMERS WERE USED. CONDITIONS USED: DENATURE (94 DEGREES CELSIUS, 3 MINUTES), 30 CYCLES (DENATURE (94 DEGREES CELSIUS, 45 SECONDS), ANNEAL (52 DEGREES CELSIUS, 45 SECONDS), EXTEND (72 DEGREES CELSIUS, 6 MINUTES)), FINAL EXTENSION (72 DEGREES CELSIUS, 10 MINUTES), AND HOLD AT 4 DEGREES CELSIUS OVERNIGHT.  
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# Named link: [http://en.wikipedia.org Wikipedia]
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Revision as of 16:15, 28 July 2009

University of Calgary

UNIVERSITY OF CALGARY



NOTEBOOK PAGE INDEX
Carol
Chinmoyee
Emily
Fahd
Iman
Jamie
Jeremy
Katie
Kevin
Mandy
Patrick
Prima
Stefan
Vicki
JULY 23, 2009 (DATE)


CAROL

AMPLIFICATION OF ''luxCDABE'' USING Taq POLYMERASE

THE PURPOSE OF THIS EXPERIMENT WAS TO AMPLIFY LUXCDABE WITH TAQ POLYMERASE. GENE SPECIFIC LUXCDABE FORWARD AND REVERSE PRIMERS WERE USED. CONDITIONS USED: DENATURE (94 DEGREES CELSIUS, 3 MINUTES), 30 CYCLES (DENATURE (94 DEGREES CELSIUS, 45 SECONDS), ANNEAL (52 DEGREES CELSIUS, 45 SECONDS), EXTEND (72 DEGREES CELSIUS, 6 MINUTES)), FINAL EXTENSION (72 DEGREES CELSIUS, 10 MINUTES), AND HOLD AT 4 DEGREES CELSIUS OVERNIGHT.


CHINMOYEE

Descriptive Title of What You're Doing

WIKI CODING HERE


EMILY

Descriptive Title of What You're Doing

WIKI CODING HERE


FAHD

Descriptive Title of What You're Doing

WIKI CODING HERE


IMAN

Descriptive Title of What You're Doing

WIKI CODING HERE


JAMIE

Descriptive Title of What You're Doing

WIKI CODING HERE


JEREMY

Descriptive Title of What You're Doing

WIKI CODING HERE


KATIE

Descriptive Title of What You're Doing

WIKI CODING HERE


KEVIN

Descriptive of What You're Doing

WIKI CODING HERE


MANDY

Descriptive Title of What You're Doing

WIKI CODING HERE


PATRICK

Descriptive Title of What You're Doing

WIKI CODING HERE


PRIMA

Descriptive Title of What You're Doing

WIKI CODING HERE


STEFAN

Descriptive Title of What You're Doing

WIKI CODING HERE


VICKI

Descriptive Title of What You're Doing

WIKI CODING HERE

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