Team:British Columbia/Project

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Revision as of 22:28, 15 May 2009

	Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Sponsor Us!	Biosensor Sensitivity Notebook	Biosensor Logic Gate Notebook	Bibliography

Overall project

1. Sensitivity of a biosensor

2. Logic gates

Biosensor

Using a random mutagenesis method directed at the promoter binding sites, we intend to construct a library of varying strength pBAD promoters coupled to a GFP reporter gene. Sequencing of select mutated promoters will result in a collection of pBAD variants that cover a wide range promoter sensitivity.

The Experiments

1. Random mutagenesis of pBAD promoter sequence. 2. Quantification of mutant promoter-driven GFP fluorescence. 3. Sequencing of select mutants. 4. BioBrick design and submission.

Results

Logic Gates

Miscellaneous Data

Biobricks.zip - Fasta file containing every biobrick from [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=List Here]

Links

http://mobyle.pasteur.fr/cgi-bin/portal.py - a set of web-accessible bioinformatics tools including Mfold, which determines 2D RNA structure and draws it

http://frodo.wi.mit.edu/ - Primer3, a primer design program

@@ Line 9: / Line 9: @@
 !align="center"|[[Team:British_Columbia/Biosensor_Sensitivity|Biosensor Sensitivity Notebook]]
 !align="center"|[[Team:British_Columbia/Biosensor_Logic_Gate|Biosensor Logic Gate Notebook]]
+!align="center"|[[Team:British_Columbia/Bibliography|Bibliography]]
 |}