Team:Calgary/26 May 2009

(Difference between revisions)

Revision as of 16:55, 28 July 2009

University of Calgary

NOTEBOOK PAGE INDEX

MAY 26, 2009

CAROL

Descriptive title of what you're doing - no need for caps

WIKI CODING HERE

EMILY

Isolation of LuxOD47E plasmids from pCR2.1-TOPO and PsB1AC3 vectors

Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.

Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations

Plasmid	260/280	230/280	Concentration [ng/μL]
LuxOD47E TOPO C1	1.88	2.21	409.5
LuxOD47E TOPO C2	1.86	2.18	551.4
LuxOD47E BBK C1	2.04	5.01	551.4
LuxOD47E BBK C2	1.77	1.39	92.9

JAMIE

Plasmid Isolation of luxOU in pCR-Blunt II-TOPO and psB1AC3

Sigma Genelute Plasmid Mini-prep kit was used. Standard manufacturer protocol used; elution in 50µL of ddH₂O.

JEREMY

Descriptive title of what you're doing - no need for caps

WIKI CODING HERE

KEVIN

Descriptive title of what you're doing - no need for caps

WIKI CODING HERE

VICKI

Descriptive title of what you're doing - no need for caps

WIKI CODING HERE

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-Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.
+*Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.
 <br>
 <br>
-Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations
+*Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations
 <br>
 <br>
-Plasmid                        260/280                           260/230                    Concentration [ng/μL]
+{|
+! Plasmid
-LuxOD47E Topo C1    1.88                                 2.21                          409.5
+! 260/280
-LuxOD47E Topo C2    1.86                                 2.18                          551.4
+! 230/280
-LuxOD47E BBK C1    2.04                                 5.01                          551.4
+! Concentration [ng/μL]
-LuxOD47E BBK C2    1.77                                 1.39                          92.9
+|-
+|LuxOD47E TOPO C1
+|1.88
+|2.21
+|409.5
+|-
+|LuxOD47E TOPO C2
+|1.86
+|2.18
+|551.4
+|-
+|LuxOD47E BBK C1
+|2.04
+|5.01
+|551.4
+|-
+|LuxOD47E BBK C2
+|1.77
+|1.39
+|92.9
+|}