From 2009.igem.org
(Difference between revisions)
|
|
| Line 178: |
Line 178: |
| | <table border="0"> | | <table border="0"> |
| | <tr border="1"> | | <tr border="1"> |
| - | <td><u>Plasmid sample</u></td> | + | <td width="150px"><u>Plasmid sample</u></td> |
| - | <td><u>260/280</u></td> | + | <td width="50px"><u>260/280</u></td> |
| - | <td><u>260/230</u></td> | + | <td width="50px"><u>260/230</u></td> |
| - | <td><u>Concentration(ng/µL)</u></td> | + | <td width="200px"><u>Concentration(ng/µL)</u></td> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
Revision as of 18:06, 28 July 2009
University of Calgary
|
|
CAROL
Descriptive title of what you're doing - no need for caps
|
|
|
EMILY
Plasmid Isolation of LuxOD47E in pCR2.1 TOPO and psB1AC3 vectors
- Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.
- Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations
| Plasmid | 260/280 | 260/230 | Concentration [ng/μL]
|
| LuxOD47E TOPO C1 | 1.88 | 2.21 | 409.5
|
| LuxOD47E TOPO C2 | 1.86 | 2.18 | 551.4
|
| LuxOD47E BBK C1 | 2.04 | 5.01 | 551.4
|
| LuxOD47E BBK C2 | 1.77 | 1.39 | 92.9
|
|
|
|
JAMIE
Plasmid Isolation of luxOU in pCR-Blunt II-TOPO and psB1AC3
Sigma Genelute Plasmid Mini-prep kit (lot # 048k6062)as used. Standard manufacturer protocol used; elution in 50µL of ddH 2O.
| Plasmid sample |
260/280 |
260/230 |
Concentration(ng/µL) |
| TOP10 luxOU C1 |
1.91 |
2.58 |
365.6 |
| TOP10 luxOU C2 |
1.90 |
2.52 |
287.2 |
| BBK luxOU C1 |
1.87 |
2.22 |
534.2 |
| BBK luxOU C2 |
1.92 |
2.24 |
715.2 |
|
|
|
JEREMY
Descriptive title of what you're doing - no need for caps
|
|
|
KEVIN
Descriptive title of what you're doing - no need for caps
|
|
|
VICKI
Descriptive title of what you're doing - no need for caps
|
"
