Team:Calgary/26 May 2009

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Sigma Genelute Plasmid Mini-prep kit (lot # 048k6062)as used. Standard manufacturer protocol used; elution in 50&#181;L of ddH<sub>2</sub>O.
Sigma Genelute Plasmid Mini-prep kit (lot # 048k6062)as used. Standard manufacturer protocol used; elution in 50&#181;L of ddH<sub>2</sub>O.
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Revision as of 18:07, 28 July 2009

University of Calgary

UNIVERSITY OF CALGARY



NOTEBOOK PAGE INDEX
Carol
Emily
Jamie
Jeremy
Kevin
Vicki
MAY 26, 2009


CAROL

Descriptive title of what you're doing - no need for caps

WIKI CODING HERE


EMILY

Plasmid Isolation of LuxOD47E in pCR2.1 TOPO and psB1AC3 vectors

  • Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.



  • Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations



Plasmid 260/280 260/230 Concentration [ng/μL]
LuxOD47E TOPO C1 1.88 2.21 409.5
LuxOD47E TOPO C2 1.86 2.18 551.4
LuxOD47E BBK C1 2.04 5.01 551.4
LuxOD47E BBK C2 1.77 1.39 92.9



JAMIE

Plasmid Isolation of luxOU in pCR-Blunt II-TOPO and psB1AC3

Sigma Genelute Plasmid Mini-prep kit (lot # 048k6062)as used. Standard manufacturer protocol used; elution in 50µL of ddH2O.

Plasmid sample 260/280 260/230 Concentration(ng/µL)
TOP10 luxOU C1 1.91 2.58 365.6
TOP10 luxOU C2 1.90 2.52 287.2
BBK luxOU C1 1.87 2.22 534.2
BBK luxOU C2 1.92 2.24 715.2


JEREMY

Descriptive title of what you're doing - no need for caps

WIKI CODING HERE


KEVIN

Descriptive title of what you're doing - no need for caps

WIKI CODING HERE


VICKI

Descriptive title of what you're doing - no need for caps

WIKI CODING HERE

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