Team:Calgary/26 May 2009

(Difference between revisions)

Revision as of 18:13, 28 July 2009

University of Calgary

NOTEBOOK PAGE INDEX

MAY 26, 2009

CAROL

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WIKI CODING HERE

EMILY

Plasmid Isolation of LuxOD47E in pCR2.1 TOPO and psB1AC3 vectors

Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.

Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations

JAMIE

Plasmid Isolation of luxOU in pCR-Blunt II-TOPO and psB1AC3

Sigma Genelute Plasmid Mini-prep kit (lot # 048k6062)as used. Standard manufacturer protocol used; elution in 50µL of ddH₂O.

JEREMY

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WIKI CODING HERE

KEVIN

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WIKI CODING HERE

VICKI

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WIKI CODING HERE

@@ Line 139: / Line 139: @@
 | '''Plasmid''' || '''260/280'''    || '''260/230''' || '''Concentration [ng/μL]'''
 |-
-| LuxOD47E TOPO C1  || 1.88 || 2.21 || 409.5
+| ''LuxO''D47E TOPO C1  || 1.88 || 2.21 || 409.5
 |-
-| LuxOD47E TOPO C2|| 1.86 ||  2.18 ||551.4
+| ''LuxO''D47E TOPO C2|| 1.86 ||  2.18 ||551.4
 |-
-| LuxOD47E BBK C1|| 2.04|| 5.01|| 551.4
+| ''LuxO''D47E BBK C1|| 2.04|| 5.01|| 551.4
 |-
-| LuxOD47E BBK C2|| 1.77|| 1.39|| 92.9
+| ''LuxO''D47E BBK C2|| 1.77|| 1.39|| 92.9
 |}