Team:Calgary/27 May 2009

From 2009.igem.org

(Difference between revisions)

Revision as of 20:25, 28 July 2009

University of Calgary

May
M	T	W	T	F	S	S
				1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30	31

NOTEBOOK PAGE INDEX

Carol
Chinmoyee
Emily
Fahd
Iman
Jamie
Jeremy
Katie
Kevin
Mandy
Patrick
Prima
Stefan
Vicki

MAY 27, 2009

CAROL

Descriptive Title of What You're Doing

WIKI CODING HERE:

bullet

bold italics underline

Named link: [http://en.wikipedia.org Wikipedia]

link to books using ISBNs
will end up like:

CHINMOYEE

Descriptive Title of What You're Doing

WIKI CODING HERE

EMILY

Descriptive Title of What You're Doing

WIKI CODING HERE

FAHD

Descriptive Title of What You're Doing

WIKI CODING HERE

IMAN

Descriptive Title of What You're Doing

WIKI CODING HERE

JAMIE

Descriptive Title of What You're Doing

WIKI CODING HERE

JEREMY

Descriptive Title of What You're Doing

WIKI CODING HERE

KATIE

Descriptive Title of What You're Doing

WIKI CODING HERE

KEVIN

Verification of Pqrr4

Restriction Digest To verify the isolated Pqrr4, restriction digest was done with NotI (invitrogen, Lot 2803027) enzymes, React Buffer 3, and GeneRuler SM1333 ladder.

Polymerase Chain Reaction (PCR) The Pqrr4 was then amplified using Polymerase Chain Reaction (PCR) from biobrick AC (pSB1AC3) using primers Pqrr4 F/R and platinum Thermus aquaticus (pTaq; invitrogen) according to the specifications of the manufacturer. The following cycling conditions were used: 94°C for 3 min; 36x (94°C for 30s; 53°C for 45s; 72°C for 1 min;) 72°C for 10 min; held at 4°C.

Sequencing Lastly, the Colony 1, being Pqrr4 BBK Green, was sent for sequencing with Pqrr4 F/R primers to the University of Calgary DNA Sequencing Facility (University Core DNA Services, Calgary, Alberta, Canada).
Sequencing needs to be added

MANDY

Descriptive Title of What You're Doing

WIKI CODING HERE

PATRICK

Descriptive Title of What You're Doing

WIKI CODING HERE

PRIMA

Descriptive Title of What You're Doing

WIKI CODING HERE

STEFAN

Descriptive Title of What You're Doing

WIKI CODING HERE

VICKI

Descriptive Title of What You're Doing

WIKI CODING HERE

@@ Line 60: / Line 60: @@
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+<br><center>
 <br>
+<br>
+<table>
+<tr>
+<td>
+<table class="month">
+<tr class="heading">
+<td colspan="7">May
+</td></tr>
+<tr class="dow">
+<td>M</td><td>T</td><td>W</td><td>T</td><td>F</td><td>S</td><td>S
+</td></tr>
+<tr>
+<td>&nbsp;
+</td><td>&nbsp;
+</td><td>&nbsp;
+</td><td>&nbsp;
+</td><td><a href="https://2009.igem.org/Team:Calgary/1_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/1_May_2009" rel="nofollow"><span class="day-active">1</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/2_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/2_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">2</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/3_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/3_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">3</span></a>
+</td></tr>
+<tr>
+<td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/4_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/4_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">4</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/5_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/5_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">5</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/6_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/6_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">6</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/7_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/7_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">7</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/8_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/8_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">8</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/9_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/9_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">9</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/10_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/10_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">10</span></a>
+</td></tr>
+<tr>
+<td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/11_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/11_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">11</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/12_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/12_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">12</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/13_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/13_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">13</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/14_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/14_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">14</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/15_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/15_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">15</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/16_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/16_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">16</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/17_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/17_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">17</span></a>
+</td></tr>
+<tr>
+<td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/18_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/18_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">18</span></a>
+</td><td><a href="https://2009.igem.org/Team:Calgary/19_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/19_May_2009" rel="nofollow"><span class="day-active">19</span></a>
+</td><td><a href="https://2009.igem.org/Team:Calgary/20_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/20_May_2009" rel="nofollow"><span class="day-active">20</span></a>
+</td><td><a href="https://2009.igem.org/Team:Calgary/21_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/21_May_2009" rel="nofollow"><span class="day-active">21</span></a>
+</td><td><a href="https://2009.igem.org/Team:Calgary/22_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/22_May_2009" rel="nofollow"><span class="day-active">22</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/23_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/23_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">23</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/24_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/24_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">24</span></a>
+</td></tr>
+<tr>
+<td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/25_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/25_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">25</span></a>
+</td><td><a href="https://2009.igem.org/Team:Calgary/26_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/26_May_2009" rel="nofollow"><span class="day-active">26</span></a>
+</td><td><a href="https://2009.igem.org/Team:Calgary/27_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/27_May_2009" rel="nofollow"><span class="day-active">27</span></a>
+</td><td><a href="https://2009.igem.org/Team:Calgary/28_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/28_May_2009" rel="nofollow"><span class="day-active">28</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/29_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/29_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">29</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/30_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/30_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">30</span></a>
+</td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/31_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/31_May_2009&amp;preload=Team:Calgary/NotebookPreload&amp;action=edit" rel="nofollow"><span class="day-empty">31</span></a>
+</td></tr></table>
+</td></tr></table>
+<p>
+</center>
 <div class="heading">NOTEBOOK PAGE INDEX</div>
 <div class="desc">
@@ Line 328: / Line 391: @@
 <br>
 <div class="heading">
-Descriptive of What You're Doing
+Verification of <i>Pqrr4</i>
 </div>
 <br>
 <div class="desc">
-</html>
+<b>Restriction Digest</B>
-WIKI CODING HERE
+To verify the isolated <i>Pqrr4</i>, restriction digest was done with NotI (invitrogen, Lot 2803027) enzymes, React Buffer 3, and GeneRuler SM1333 ladder.
+<br>
+<br>
+<b>Polymerase Chain Reaction (PCR) </b>
+The <i>Pqrr4</i> was then amplified using   Polymerase Chain Reaction (PCR) from biobrick AC (pSB1AC3) using primers Pqrr4 F/R and platinum Thermus aquaticus (pTaq; invitrogen) according to the specifications of the manufacturer. The following cycling conditions were used:
+°C for 3 min; 36x (94°C for 30s; 53°C for 45s; 72°C for 1 min;) 72°C for 10 min; held at 4°C.
+<br>
-<html>
+<br>
+<b> Sequencing </b>
+Lastly, the Colony 1, being <i>Pqrr4</i> BBK Green, was sent for sequencing with Pqrr4 F/R primers to the University of Calgary DNA Sequencing Facility (University Core DNA Services, Calgary, Alberta, Canada).
+<br>
+Sequencing needs to be added
+</p><p><br />
 </div>
 </td>