From 2009.igem.org
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- | Descriptive Title of What You’re Doing
| + | Modeling Readings Continued |
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- | WIKI CODING HERE
| + | * Continued with reading up on things to characterize for the AI-2 system |
| + | * Started familiarization with Single Site-Directed Mutagenesis protocol |
| + | * Primers for mutagenesis is ordered. The following are the sequences for the mutagenesis: |
| + | LuxCDABE-M-F: CCATTAATGAATTGCCGGATAATCTGGATTTTGAAGGCC |
| + | LuxCDABE-M-R: GGCCTTCAAAATCCAGATTATCCGGCAATTCATTAATGG |
| + | * Both primers are PAGE purified. |
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Revision as of 23:52, 28 July 2009
University of Calgary
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CAROL
Modeling Readings Continued
- Continued with reading up on things to characterize for the AI-2 system
- Started familiarization with Single Site-Directed Mutagenesis protocol
- Primers for mutagenesis is ordered. The following are the sequences for the mutagenesis:
LuxCDABE-M-F: CCATTAATGAATTGCCGGATAATCTGGATTTTGAAGGCC
LuxCDABE-M-R: GGCCTTCAAAATCCAGATTATCCGGCAATTCATTAATGG
- Both primers are PAGE purified.
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CHINMOYEE
Descriptive Title of What You're Doing
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EMILY
Sequencing of LuxOD47E in psB1AC3
- Objective: To veriffy the presence of the LuxOD47E gene in the psB1AC3 BBK vector
Size of plasmid Backbone: 3055 bp
Size of the gene of interest: 1362 bp
Total size: 4417 bp
Sequencing needs 100ng of DNA/ 1 kb
4.4kb x 100ng/kb = 440ng
Concentration of C2= 92.9ng/μL
440ng/ 92.9 ng/uL = 4.8 μL DNA
Diluted 4.8 μL DNA (LuxOD47E BBK C2) with 5.2 μL ddH20 and sent down to Univeristy of Calgary Sequencing Labs with VF2 and VF1 primers.
- Results: Sequencing came back and results indicated that the gene if interest, LuxOD47E was not in the psB1AC3 vector. We will have to go back to the gene in the pCR2.1-TOPO vector and try to get it into the psB1AC3 vector.
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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