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Team:Calgary/17 July 2009
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| - | + | Ligation of promoter library and pCS26 and Transformation of plasmid in ''E.coli'' | |
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| - | + | * Following the overnight enzyme digestion (BamHI and XhoI) of the vector (pCS26) and promoter (created by the 32 different primers), the vector (pCS26) was treated with anarctic phosphatase for 30 minutes (see proper protocol in procedure page) and the products was ligated with 3 different methods: | |
| + | 1. NEB Quick Ligase for 5 minutes | ||
| + | 2. Invitrogen T4 Ligase at 16<sup>o</sup>C overnight | ||
| + | 3. Invitrogen T4 Ligase at room temperature (~21<sup>o</sup>C) for one week | ||
| + | * The Quick Ligated product was transformed into Top 10 cells and was grown on LB plates with Kan resistance for 16 hours. | ||
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Revision as of 03:04, 29 July 2009
UNIVERSITY OF CALGARY
