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Team:Calgary/16 July 2009
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| - | + | Diluting Primers and Primer Extension Polymerase Chain Reaction (PCR) | |
</div> | </div> | ||
<br> | <br> | ||
<div class="desc"> | <div class="desc"> | ||
</html> | </html> | ||
| - | + | * To create the synthetic sigma 70 promoter library, we have synthesized 32 different primers (16 different forward primers and 16 different reverse primers) to hopefully form 256 different promoters. | |
| + | * Need to dilute primers to 80μM. The following is the concentration of the original primers that were synthesized once water was added to the primers. | ||
| + | <center> | ||
| + | {| border="1" | ||
| + | | '''Primer''' || '''260/280''' || '''260/230''' || '''Concentration [ng/μL]''' || '''Reading/2.5 [ng/μL]''' || '''Stock Concentration [μM]''' || '''Volume stock into working [μL]''' || '''Volume water into working [μL]''' || | ||
| + | |- | ||
| + | | ''sig70-F1 || 1.98 || 1.73 || 20816 || 832.64 || 60.07 || 6.7 || 43.3 | ||
| + | |- | ||
| + | | ''sig70-F2 || 1.55 || 1.80 || 44284 || 17713.6 || 1277.91 || 3.1 || 46.9 | ||
| + | |- | ||
| + | | ''psB1AC3 3 || 1.87|| 1.88|| 48.5 | ||
| + | |- | ||
| + | | ''psB1AC3 4|| 1.90|| 2.11|| 118.9 | ||
| + | |- | ||
| + | | ''psB1AK3 1 || 2.05 || 2.42 || 134.4 | ||
| + | |- | ||
| + | | ''psB1AK3 2 || 1.95 || 2.23 || 55.6 | ||
| + | |- | ||
| + | | ''psB1AK3 3 || 1.91 || 2.22 || 97.9 | ||
| + | |- | ||
| + | | ''psB1AK3 4 || 2.03 || 1.92 || 44.5 | ||
| + | |} | ||
| + | </center> | ||
| + | |||
<html> | <html> | ||
</div> | </div> | ||
Revision as of 04:11, 29 July 2009
UNIVERSITY OF CALGARY
