Team:Calgary/2 July 2009
From 2009.igem.org
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*Protocol: Used GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions, eluting in 50 μL ddH2O. | *Protocol: Used GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions, eluting in 50 μL ddH2O. | ||
- | *Results | + | *Results |
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Reaction 1- Not1 + React 3 Buffer | Reaction 1- Not1 + React 3 Buffer | ||
+ | <Br> | ||
Reaction 2- EcoRI + SpeI + React Buffer 4 | Reaction 2- EcoRI + SpeI + React Buffer 4 | ||
+ | <Br> | ||
Reaction 3- XbaI + PstI + React 2 Buffer | Reaction 3- XbaI + PstI + React 2 Buffer | ||
+ | <Br> | ||
Reaction 4- EcoRI + PstI + React 2 Buffer | Reaction 4- EcoRI + PstI + React 2 Buffer | ||
+ | <Br> | ||
Reaction 5- XbaI + SpeI + React 4 Buffer | Reaction 5- XbaI + SpeI + React 4 Buffer | ||
+ | <Br> | ||
<Br> | <Br> | ||
BBK vector tube preparation | BBK vector tube preparation |
Revision as of 19:36, 29 July 2009
UNIVERSITY OF CALGARY