Minnesota/1 July 2009
From 2009.igem.org
(Difference between revisions)
Line 5: | Line 5: | ||
|'''[[Minnesota/30 June 2009|Go to Previous Day (June 30)]]'''|| width=158|'''[[Minnesota/2 July 2009|Go to Next Day (July 2)]]''' | |'''[[Minnesota/30 June 2009|Go to Previous Day (June 30)]]'''|| width=158|'''[[Minnesota/2 July 2009|Go to Next Day (July 2)]]''' | ||
|} | |} | ||
- | <br> | + | '''Patrick'''<br> |
+ | A major issue was uncovered with the model being used. Specifically, designGUI, when deleting reactions, will delete unused species, which is good. The GUI though also does not update which species number is being "Split on Division" (SOD). This resulted in RNAp being split every time the cells divided. Since it wasn't being produced, the number of RNAp per cell would continue to decrease as time went on, which would result in lower GFP production than expected.<br> | ||
+ | |||
+ | Today I ran the base model after checking to make sure, finally, that everything was as it was expected to be, including the species being split on division:<br> | ||
+ | '''Ben'''<br> | ||
[[Image:Tnnatcvariesleaky15.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br> | [[Image:Tnnatcvariesleaky15.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br> |
Revision as of 15:20, 30 July 2009
Back to Notebook Home | |
Go to Previous Day (June 30) | Go to Next Day (July 2) |
Patrick
A major issue was uncovered with the model being used. Specifically, designGUI, when deleting reactions, will delete unused species, which is good. The GUI though also does not update which species number is being "Split on Division" (SOD). This resulted in RNAp being split every time the cells divided. Since it wasn't being produced, the number of RNAp per cell would continue to decrease as time went on, which would result in lower GFP production than expected.
Today I ran the base model after checking to make sure, finally, that everything was as it was expected to be, including the species being split on division:
Ben