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| - | <div class="heading">NOTEBOOK PAGE INDEX</div>
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| - | <div class="desc">
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| - | <a href="#Carol">Carol</a><br>
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| - | <a href="#Chinmoyee">Chinmoyee</a><br>
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| - | <a href="#Emily">Emily</a><br>
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| - | <a href="#Fahd">Fahd</a><br>
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| - | <a href="#Iman">Iman</a><br>
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| - | <a href="#Jamie">Jamie</a><br>
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| - | <a href="#Jeremy">Jeremy</a><br>
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| - | <a href="#Katie">Katie</a><br>
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| - | <a href="#Kevin">Kevin</a><br>
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| - | <a href="#Mandy">Mandy</a><br>
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| - | <a href="#Patrick">Patrick</a><br>
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| - | <a href="#Prima">Prima</a><br>
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| - | <a href="#Stefan">Stefan</a><br>
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| - | <a href="#Vicki">Vicki</a><br>
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| - | </div>
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| - | </p></div>
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| - | <div class="heading">CALENDAR</div>
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| - | <div class="desc">
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| - | <center>
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| | </html> | | </html> |
| - | {| style="background:transparent;" | + | {{Template:CalgaryNotebook}} |
| - | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=05 | title=Team:Calgary}}
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| - | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=07 | title=Team:Calgary}}
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| - | |}
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| - | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=08 | title=Team:Calgary}}
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| - | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=10 | title=Team:Calgary}}
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| | <center> | | <center> |
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CAROL
Modeling Readings Continued
- Continued with reading up on things to characterize for the AI-2 system
- Started familiarization with Single Site-Directed Mutagenesis protocol
- Primers for mutagenesis is ordered. The following are the sequences for the mutagenesis:
LuxCDABE-M-F:CCATTAATGAATTGCCGGATAATCTGGATTTTGAAGGCC
LuxCDABE-M-R:GGCCTTCAAAATCCAGATTATCCGGCAATTCATTAATGG
- Both primers are PAGE purified.
- Received licences for Matlab and Simbiology. Downloaded program and was able to practice through examples found in the Matlab package. Focused on G protein cascase model (example in Matlab) in Yeast and prepared a presentation to show other iGEM members how Simbiology works.
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CHINMOYEE
Descriptive Title of What You're Doing
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EMILY
Sequencing of LuxOD47E in psB1AC3
- Objective: To veriffy the presence of the LuxOD47E gene in the psB1AC3 BBK vector
Size of plasmid Backbone: 3055 bp
Size of the gene of interest: 1362 bp
Total size: 4417 bp
Sequencing needs 100ng of DNA/ 1 kb
4.4kb x 100ng/kb = 440ng
Concentration of C2= 92.9ng/μL
440ng/ 92.9 ng/uL = 4.8 μL DNA
Diluted 4.8 μL DNA (LuxOD47E BBK C2) with 5.2 μL ddH20 and sent down to Univeristy of Calgary Sequencing Labs with VF2 and VF1 primers.
- Results: Sequencing came back and results indicated that the gene if interest, LuxOD47E was not in the psB1AC3 vector. We will have to go back to the gene in the pCR2.1-TOPO vector and try to get it into the psB1AC3 vector.
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FAHD
Descriptive Title of What You're Doing
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IMAN
Descriptive Title of What You're Doing
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JAMIE
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JEREMY
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KATIE
Descriptive Title of What You're Doing
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KEVIN
Matlab and symbiology
Familiarizing with the matlab program provided by MathWorks, and its biological tool called Symbiology.
No experiments were done on this day.
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MANDY
Descriptive Title of What You're Doing
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PATRICK
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PRIMA
Descriptive Title of What You're Doing
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STEFAN
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VICKI
Descriptive Title of What You're Doing
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"
